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KMID : 1098420060140040195
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 4 p.195 ~ p.201
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Fibrinolytic Enzyme Activity of Extract from Camellia japonica L.
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Lim Chae-Young
Lee Sook-Young
Pyo Byoung-Sik
Kim Sun-Min
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Abstract
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The fibrinolytic activities of soluble proteins extracted from young leaves of Camellia japonica L. were studied. Fibrinolvity activity of extract from partitions of C. japonica L. showed 1.6-2.0 times higher than plasmin used as positive control. The fibrinolytic enzyme was confirmed directly from young leaves of C. japonica L. by a fibrin Plate and fibrin zymography. The protein was composed of a single polypeptide and its apparent molecular weight was found to be 45 kDa, as judged by SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature for the fibrinolytic activity were pH 5.5 and 30^{circ}C, respectively. Also, the fibrinolytic activity was clearly inhibited by PMSF and TLCK, suggesting that it is a member of the trypsin-like serine protease. All these results suggest the protease is a fibrinolytic enzyme belong to a family of trypsin-like serine protease.
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KEYWORD
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Camellia japonica L, fibrinolytic enzyme, Protease activity, fibrinolytic activity, fibrin zymography
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